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Biological Materials Technologies

Researchers at the University of Florida have developed numerous monoclonal antibodies. Many of the antibodies available for licensing are listed below. To meet the needs of our commercial partners for fast, convenient access to these technologies, we have developed a standard non-exclusive license.

For more information on these or other monoclonal antibodies, please contact Melanie Campos or John Byatt at the UF Office of Technology Licensing, (352) 392-8929.

Monoclonal Antibodies

PDF ID Lead Inventor Title
  12970 Chan, Edward Kar Leung Monoclonal antibodies against TNGW1 (Trinucleotide GW1)

RNA interference (RNAi), triggered by small interfering RNA and microRNA (miRNA), is a potent mechanism in post-transcriptional regulation for gene expression. GW182 is a 182kDa protein which binds to the 3’ untranslated region of mRNA targeted by miRNA and resulting in translation suppression. Trinucleotide GW1 (TNGW1) is a novel 210kDa isoform of human GW182; so named because it contains trinucleotide repeats in the mRNA sequence. TNGW1 expresses independently of GW182 and is present in human testes and various human cancer cells.
  11731 Battelle, Barbara Anne Monoclonal antibodies against Limulus Visual Arrestin

MAb (4 different hybridomas) raised against the N-terminal half of recombinant Limulus visual arrestin. Antibodies are specific for Limulus, but wide species cross-reactivity was not tested.
  11934 Campbell-Thompson, Martha Monoclonal antibody against Cholecystokinin (CCK) Receptor 2

This antibody was generated against recombinant rat CCKB receptor. The antibody cross-reacts with mouse CCK2 as well as the human homolog (92% amino acid similarity) and has been used to show positive immuno-reactivity to cortical neurons via immunohistochemistry. This antibody is expected to be useful for studies in the central nervous system, gastrointestinal tract and other organs.
  11150 Chan, Edward Kar Leung Monoclonal and Polyclonal antibodies for GW Protein Rich Bodies (cytoplasmic compartment regulating mRNA degradation)

Transcription is important for regulating gene expression in most cell types. However, other mechanisms that regulate the stability or lability of mRNAs are also important in controlling gene expression. There are several novel mRNA binding molecules that are specific markers for a cytoplasmic mRNA compartment where mRNA is stored and may be destined for degradation via the LSm complex pathway. One of these binding proteins is GW1 (aka GW182) which appears to be a highly specific marker for the cytoplasmic compartment GW protein rich bodies. The specific antibodies allow examination of this mRNA compartments in real time.
  12095a Chan, Edward Kar Leung Monoclonal antibody against Argonaute-2 (endonuclease within the RNA-induced silencing complex)

Argonaute-2 (Ago2) is an endonuclease within the RNA-induced silencing complex (RISC) and uses small interfering RNA (siRNA) as a guide to find mRNA containing complementary sequences. Monoclonal antibodies were generated using the full length recombinant human protein as antigen.
  12095b Chan, Edward Kar Leung Monoclonal antibody against P62

P62 is a cytoplasmic mRNA binding protein which binds to mRNA encoding IGF-II and may be involved in hepatic carcinogenesis because autoantibodies are found in a large percentage of patients with hepatocellular carcinoma. MAb were generated using the full length recombinant human protein as antigen.
  12095c Chan, Edward Kar Leung Monoclonal antibody against P90

P90 is a previously unidentified cytoplasmic protein that appears to have been conserved across species, but has no significant homology to any known gene products. It appears to be a companion auto-antigen to p62 and may represent a new marker for tumors such as hepatocellular carcinoma, gastric cancer and prostate cancer because autoantibodies are found in a large percentage of patients with these cancers. MAb were generated using the N-terminal third of the recombinant human protein as antigen.
  12095d Chan, Edward Kar Leung Monoclonal antibody against GW3

GW3 is a cellular protein closely related to GW182 protein. GW182 is a critical component of GW bodies which are small cytoplasmic foci found in proliferating cells and are involved in post-transcriptional regulation of gene expression. MAb were generated using the full length recombinant human GW3 protein as antigen.
  11075 Marsh, Robert Monoclonal antibody against human Recoverin

Recoverin is a neurologically important calcium binding protein, conserved across species, with an approximate molecular weight of 23kDa. There are also indications of several isoforms of this molecule. The antibodies which cross react with human recoverin and an extract of mouse brain were found to recognize the pineal region of the human brain.
  12246 May, Stratford A monoclonal antibody that specifically recognizes phosphorylated RAX/PACT protein

Murine RAX (PKR Activator X) protein and its human ortholog PACT (Protein Activator of Interferon-induced Protein Kinase) are the only known cellular activators of the interferon-induced dsRNA-dependent protein kinase, PKR. RAX and PACT are 98% identical in amino acid sequence. In addition, RAX/PACT associates with Dicer to form the RNA-induced silencing complex (RISC) and promote small RNA-mediated gene silencing. RAX is phosphorylated on serine 18 during cellular stress applications and this phosphorylation is required for PKR activation and consequent translation inhibition during events such as growth factor deprivation, cytotoxic cytokine or chemotherapy treatment and viral infection. In addition, exogenous expression of RAX(S18A) promotes anchorage-independent growth of fibroblast cell lines, indicating that RAX phosphorylation is required for normal cellular growth regulation. A peptide composed of RAX amino acids 13 to 25, where phosphoserine was substituted for serine at position 18, was used as antigen to generate a hybridoma cell line that produces monoclonal antibody specific to phosphorylated RAX.
  12247 May, Stratford A monoclonal antibody that specifically recognizes RAX/PACT protein

Murine RAX (PKR Activator X) protein and its human ortholog PACT (Protein Activator of Interferon-induced Protein Kinase) are the only known cellular activators of the interferon-induced dsRNA-dependent protein kinase, PKR. RAX and PACT are 98% identical in amino acid sequence. In addition, RAX/PACT associates with Dicer to form the RNA-induced silencing complex (RISC) and promote small RNA-mediated gene silencing. RAX is phosphorylated on serine 18 during cellular stress applications and this phosphorylation is required for PKR activation and consequent translation inhibition during events such as growth factor deprivation, cytotoxic cytokine or chemotherapy treatment and viral infection. In addition, exogenous expression of RAX(S18A) promotes anchorage-independent growth of fibroblast cell lines, indicating that RAX phosphorylation is required for normal cellular growth regulation. Two peptides (corresponding to RAX amino acids 13 to 25, but one peptide contained phosphoserine instead of serine at position 18) were used as antigens to generate a hybridoma cell line producing a monoclonal antibody that recognizes both non-phosphorylated and phosphorylated RAX.
  11931 Miller, Tyler Monoclonal antibody against human Calcium-Sensing Receptor

Mouse monoclonal antibody directed against the extracellular N-terminus of the human calcium-sensing receptor. The antibody was generated against a synthetic peptide (SSAYGPDQRAQKK) corresponding to amino acids 15 - 29 in the extracellular amino terminus of human CaR. The antibody recognizes human, mouse, rat, and rabbit proteins.
10013 Muir, David Monoclonal antibodies against Matrix Metaloproteinase-2 (MMP-2)

This monoclonal antibody was raised against activated recombinant human MMP-2 and recognizes both pro and active MMP-2 from humans rats and mice. Abnormal expression of matrix metalloproteinases is often associated with loss of negative growth regulation and high invasive potential in neoplastic tissue. MMP-2 will split laminin-5 to expose the integrin-binding site on this component of the epithelial basement membrane which is the signalling mechanism for cells to begin migration.
10683 Muir, David Monoclonal antibody against Neurofibromin

Mouse monoclonal antibody directed against the extracellular N-terminus of the human calcium-sensing receptor. The antibody was generated against a synthetic peptide (SSAYGPDQRAQKK) corresponding to amino acids 15 - 29 in the extracellular amino terminus of human CaR. The antibody recognizes human, mouse, rat, and rabbit proteins.
  11359 Ostrov, David Monoclonal antibody against HKE2: A Novel Tumor Antigen

Prefoldin 6 is a subunit of a hexameric molecular chaperone complex, named prefoldin. This chaperon complex is built from two related classes of subunits and is present in all eukaryotes and archaea. The prefoldin complex interacts with nascent polypeptide chains to stabilize non-native proteins (such as actins and tubulins) for subsequent folding in the central cavity of a chaperonin. The prefoldin 6 gene is located in the human MHC class II region and is also known as HKE2.
10452 Swanson, Maurice Monoclonal antibodies against Heterogeneous Nuclear Ribonuclear Proteins (hnRNP) m3/4 & m1-4
  11365 Swanson, Maurice Monoclonal antibodies against yeast RNA- and DNA-Binding Proteins (e.g. PUB1, PAB1, NAB2p)
  11977 Swanson, Maurice Monoclonal antibody against CUGBP2/ETR-3 Proteins

CUGBP2/ETR-3 is member of the CELF family of pre-mRNA alternative splicing factors which play an important role in the appearance of specific protein isoforms during postnatal development. The CELF family has also been implicated in the neuromuscular disease myotonic dystrophy. The monoclonal antibody was generated against a purified human CUGBP2/ETR-3 protein fragment fused to GST and recognizes both human and mouse CUGBP2/ETR-3 proteins (elav-type RNA-binding protein), but does not bind to other members of the CELF family.

Click here for entire list and additional details on Biological Materials listed above.

Polyclonal Antibodies

PDF ID Lead Inventor Title
  10684 Muir, David Polyclonal antibody to Neurofibromin

A rabbit polyclonal antibody raised against the neurofibromatosis type 1 (NF1) gene product, neurofibromin. Antiserum was raised against a unique peptide corresponding to peptides 27-41 of the N-terminal sequence of mammalian neurofibromin. Serum was harvested and antibody to neurofibromin was isolated by affinity chromatography against the original peptide. Polyclonal antibody NFn27a binds to human and rodent neurofibromin in various biochemical assays including Western immunoblotting and immunohistochemistry (including paraffin sections). Unlike the other antibodies available to neurofibromin, NF27a does not require antigen retrieval methods for immunolabeling of paraffin sections.
  10618 Muir, David Polyclonal antiserum to rat Laminin

A rabbit polyclonal antiserum, raised against laminin protein isolated from rat heart. Laminins are a family of extracellular matrix proteins found in basement membranes and associated with cell surfaces. This antiserum recognizes the laminin-1 and laminin-2 in human and rodent species (and perhaps others) and is useful in ELISA, Western blotting and immunocytochemistry (including paraffin sections).
  11900 Robertson, Keith Polyclonal antibody to DNA Methyltransferase (DNMT1)

A rabbit polyclonal antibody raised against DNA methyltransferase 1 (DNMT1). This antibody is useful for detecting DNMT1 in western blotting, immunofluorescence, and for immunoprecipitation.

Cell Lines and Tissue Samples

PDF ID Lead Inventor Title
  12276 Muir, David Human Schwann Cell-Like: Cell line sNF02.2

NF1 Status: The MPNST culture sNF02.2 was derived from tumor material from a 35 year-old male NF1 patient who met diagnostic criteria. The originative tumor tissue for the sNF02.2 cell line was obtained from a lung metastasis diagnosed by histopathology as a MPNST. Cyogenetic analysis and genotyping data is ongoing and will be provided at a later date. The portion of the tumor specimen used for tissue culture was independently characterized by immunohistopathology and confirmed as MPNST. The sNF02.2 tumor culture was established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population and displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75. Western immunoblotting showed apparently full-length neurofibromin. Additional features of this cell line were described in recent papers Li et al., Oncogene. 23: 1146-52, 2004; Fieber et al., Neurobiol. Disease 13:136-146, 2003.
  12277 Muir, David Human Schwann Cell-Like: Cell line sNF94.3

NF1 Status: The MPNST culture sNF94.3 was derived from tumor material from a 43 year-old female NF1 patient who met diagnostic criteria. Although there was no family history, the patient had definite features of NF1 including a mild learning disability, scoliosis, café-au-lait spots, Lisch nodules, hundreds of dermal neurofibromas, a congenital plexiform in the ankle and foot, and a MPNST in the thigh. The originative tumor tissue for the sNF94.3 cell line was obtained from a lung metastasis diagnosed by histopathology as a MPNST. The portion of the tumor specimen used for tissue culture was independently characterized by immunohistopathology and confirmed as MPNST. The sNF94.3 tumor culture was established from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population and displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75. sNF94.3 has a normal karyotype, and the germline and somatic NF1 mutations remain unknown despite analysis of numerous exons. Interestingly, the LOH analysis was uninformative, showing homozygous markers across the NF1 gene. Thus, there may be a germline microdeletion in the region, which would be consistent with the patient's heavy dermal tumor burden and occurrence of the MPNST (De Raedt et al., 2003). These data predict this cell line should produce full-length neurofibromin protein. Western immunoblotting showed apparently full-length neurofibromin, confirmed with several anti-neurofibromin antibodies (Perrin et al., manuscript in press). Abnormalities in sNF94.3's neurofibromin remain unknown. Additional features of this cell line were described in recent papers Li et al., Oncogene. 23: 1146-52, 2004; Fieber et al., Neurobiol. Disease 13:136-146, 2003.
  12278 Muir, David Human Schwann Cell-Like: Cell line sNF96.2

NF1 Status: The MPNST culture sNF96.2 was derived from tumor material from a 28 year-old male NF1 patient who met diagnostic criteria. The originative tumor tissue for the sNF96.2 cell line was obtained from a recurrent mass associated with nerve in the lower leg diagnosed by histopathology as a MPNST. The portion of the tumor specimen used for tissue culture was independently characterized by immunohistopathology and confirmed as MPNST. The sNF96.2 tumor culture cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population and displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75. sNF96.2 has an abnormal karyotype, complete LOH (no detection of the remaining NF1 allele) and an identified NF1 germline was identified as a base pair deletion in exon 21 (3683delC) causing a frameshift which leads to a premature stop codon prior to the ras-GAP domain (Perrin et al., manuscript submitted). In addition, somatically this tumor has LOH of the entire 17 homolog (referred to as MPNST 459T1 in Rasmussen et al. 2000, Genes Chromosomes Cancer 28:425-431). As predicted by genotyping, the sNF96.2 culture showed no full-length neurofibromin when analyzed by Western immunoblotting using polyclonal antibodies against neurofibromin (Perrin et al., manuscript submitted). Additional features of this cell line were described in recent papers Li et al., Oncogene. 23: 1146-52, 2004; Fieber et al., Neurobiol. Disease 13:136-146, 2003.
  11328 Muir, David Tissue Samples and Sections of Neurofibromatosis Tumors